Part:BBa_K5160006
p19
Overview
A silencing repressor derived from TBSV inhibits intercellular movement of RNA silencing in plants.
Plants have gene silencing, an antiviral defense mechanism that produces siRNA to degrade viral RNA. p19 protein, as a silencing repressor of viruses, binds to siRNA and prevents the binding and degradation of viral RNA by siRNA, which allows viruses to replicate and propagate in host cells.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 142
Illegal BsaI.rc site found at 493
Biology
Plant viral expression vectors have been widely used in the regulation of plant gene expression and functional analysis. TRV is a double-stranded RNA virus, TRV1 contains the genetic information of the virus, TRV2 contains the gene encoding the viral capsid protein, and there is an MCS sequence on TRV2 for cloning. Under the action of reverse transcriptase, the genome is reverse-transcribed into cDNA in vitro, and the cDNA is specifically modified and cloned to turn it into a vector that is not virulent but has both the ability to self-replicate and express exogenous genes. Subsequently, we combined TRV2 with sweet protein and injected it into tomato together with TRV1. When the virus replicates autonomously in tomato cells, it can utilize the material in tomato cells to express the sweet protein.
At the same time, we suppressed the tomato plant's own RNA interference system by introducing the silencing repressor p19 to ensure that the viral vector could remain in the tomato for a period of time.
Design
In carrying out the design, we ensured that the viral vector could remain in tomato for a period of time by introducing the silencing suppressor P19 to inhibit the tomato plant's own RNA interference system. We deliberately constructed the p19 protein on the TRV2_35S promoter_p19_NOS terminator.
Source
The p19 protein is derived from Tombusvirus (TBSV), which can inhibit the RNA silencing effect of exogenous genes in the host, increase the stability of transcripts of heterologous genes, and then promote the expression of heterologous proteins. p19 protein is widely used in transgenic plants and transient expression systems in tobacco leaves, Arabidopsis leaves, tomato leaves or protoplasts.
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